abstract：:UDP-glucose dehydrogenase (UGDH) catalyzes oxidation of UDP-glucose to yield UDP-glucuronic acid, a precursor of hyaluronic acid (HA) and other glycosaminoglycans (GAGs) in extracellular matrix. Although association of extracellular matrix with cell proliferation and migration has been well documented, the importance ...

journal_title：Experimental cell research

authors： Wang TP,Pan YR,Fu CY,Chang HY

更新日期：2010-10-15 00:00:00

abstract：:MIST1 is a transcription factor expressed in pancreatic acinar cells and other serous exocrine cells. Mice harboring a targeted deletion of the Mist1 gene (Mist1(-/-)) exhibit alterations in acinar regulated exocytosis and aberrant Ca(2+) signaling that are normally controlled by acinar cell Ca(2+)-ATPases. Previous s...

journal_title：Experimental cell research

authors： Garside VC,Kowalik AS,Johnson CL,DiRenzo D,Konieczny SF,Pin CL

更新日期：2010-10-15 00:00:00

abstract：:Skeletal muscle differentiation is strongly inhibited by transforming growth factor type beta (TGF-beta), although muscle formation as well as regeneration normally occurs in an environment rich in this growth factor. In this study, we evaluated the role of intracellular regulatory Smads proteins as well as TGF-beta-r...

journal_title：Experimental cell research

authors： Droguett R,Cabello-Verrugio C,Santander C,Brandan E

更新日期：2010-09-10 00:00:00

abstract：:Despite an increasing interest in the role of the p53 tumour suppressor protein in embryonic stem cells, not much is known about its regulation in this cell type. We show that the relatively high amount of p53 protein correlates with a higher amount of p53 RNA in ES cells compared to differentiated cells. Moreover, p5...

journal_title：Experimental cell research

authors： Solozobova V,Blattner C

更新日期：2010-09-10 00:00:00

abstract：:Endocytosis of signaling receptors, such as epidermal growth factor receptor (EGFR), tightly controls the signal transduction process triggered by ligand activation of these receptors. To identify new regulators of the endocytic trafficking of EGFR an RNA interference screen was performed for genes involved in ubiquit...

journal_title：Experimental cell research

authors： Duex JE,Mullins MR,Sorkin A

更新日期：2010-08-01 00:00:00

abstract：:Resistance of cancer cells to apoptosis is dependent on a balance of multiple genetic and epigenetic mechanisms, which up-regulate efficacy of the surviving growth factor-receptor signaling pathways and suppress death-receptor signaling pathways. The Insulin-like Growth Factor-1 Receptor (IGF-1R) signaling pathway is ...

journal_title：Experimental cell research

authors： Karasic TB,Hei TK,Ivanov VN

更新日期：2010-07-15 00:00:00

abstract：:Extraocular muscles (EOMs) are categorized as skeletal muscles; however, emerging evidence indicates that their gene expression profile, metabolic characteristics and functional properties are significantly different from the prototypical members of this muscle class. Gene expression profiling of developing and adult ...

journal_title：Experimental cell research

authors： Moncman CL,Andrade FH

更新日期：2010-07-15 00:00:00

abstract：:Eps8 controls actin dynamics directly through its barbed end capping and actin-bundling activity, and indirectly by regulating Rac-activation when engaged into a trimeric complex with Eps8-Abi1-Sos1. Recently, Eps8 has been associated with promotion of various solid malignancies, but neither its mechanisms of action n...

journal_title：Experimental cell research

authors： Welsch T,Younsi A,Disanza A,Rodriguez JA,Cuervo AM,Scita G,Schmidt J

更新日期：2010-07-15 00:00:00

abstract：:Hypoxia inducible factor 1 (HIF-1), the key mediator of hypoxia signaling pathways, has been shown involved in hypoxia-induced radioresistance. However, the underlying mechanisms are unclear. The present study demonstrated that both hypoxia and hypoxia mimetic cobalt chloride could increase the radioresistance of huma...

journal_title：Experimental cell research

authors： Liu J,Zhang J,Wang X,Li Y,Chen Y,Li K,Zhang J,Yao L,Guo G

更新日期：2010-07-15 00:00:00

abstract：:12-Lipoxygenase, an arachidonic acid metabolizing enzyme of the lipoxygenase pathway, has been implicated as a major factor in promoting prostate cancer progression and metastasis. The ability of 12-LOX to aggravate the disease was linked to its proangiogenic role. Recent studies clearly demonstrated that 12-LOX enhan...

journal_title：Experimental cell research

authors： Krishnamoorthy S,Jin R,Cai Y,Maddipati KR,Nie D,Pagès G,Tucker SC,Honn KV

更新日期：2010-06-10 00:00:00

abstract：:In the trabecular meshwork (TM) of the eye, regulation of tissue contractility by the PPRARI sequence within the Heparin II (HepII) domain of fibronectin is believed to control the movement of aqueous humor and dictate the level of intraocular pressure. This study shows that the HepII domain utilizes activated alpha4b...

journal_title：Experimental cell research

authors： Schwinn MK,Gonzalez JM Jr,Gabelt BT,Sheibani N,Kaufman PL,Peters DM

更新日期：2010-05-15 00:00:00

abstract：:We have earlier shown that mouse pancreatic acinar cells produce hydrogen sulfide (H(2)S), which plays a key role in the pathogenesis of acute pancreatitis (AP). H(2)S-dependent induction of inflammation is mediated by the activation of transcription factor NF-kappaB. We now provide evidence that activation of Src fam...

journal_title：Experimental cell research

authors： Tamizhselvi R,Koh YH,Sun J,Zhang H,Bhatia M

更新日期：2010-05-15 00:00:00

abstract：:Rhabdomyosarcoma (RMS) are soft-tissue sarcoma commonly encountered in childhood. RMS cells can acquire invasive behavior and form metastases. The metastatic dissemination implicates many proteases among which are mu-calpain and m-calpain. Study of calpain expression and activity underline the deregulation of calpain ...

journal_title：Experimental cell research

authors： Roumes H,Leloup L,Dargelos E,Brustis JJ,Daury L,Cottin P

更新日期：2010-05-15 00:00:00

abstract：:In this study we demonstrated that neural rosettes derived from human ES cells can give rise either to neural crest precursors, following expansion in presence of bFGF and EGF, or to dopaminergic precursors after exposure to ventralizing factors Shh and FGF8. Both regionalised precursors are capable of extensive proli...

journal_title：Experimental cell research

authors： Colleoni S,Galli C,Giannelli SG,Armentero MT,Blandini F,Broccoli V,Lazzari G

更新日期：2010-04-15 00:00:00

abstract：:Raver2 was originally identified as a member of the hnRNP family through database searches revealing three N-terminal RNA recognition motifs (RRMs) bearing highest sequence identity in the RNP sequences to the related hnRNP Raver1. Outside the RRM region, both Raver proteins are quite divergent in sequence except for ...

journal_title：Experimental cell research

authors： Henneberg B,Swiniarski S,Sabine Becke,Illenberger S

更新日期：2010-04-01 00:00:00

abstract：:CEACAM1-4S (carcinoembryonic antigen-related cell adhesion molecule 1) is a type I membrane protein with a short (12-amino acid) cytoplasmic tail. Wild type CEACAM1-4S-transfected MCF7 cells form glands with lumena when grown in 3D culture, while null mutations of two putative phosphorylation sites (T457A and S459A) i...

journal_title：Experimental cell research

authors： Chen CJ,Nguyen T,Shively JE

更新日期：2010-02-15 00:00:00

abstract：:In confluent keratinocyte monolayers, desmosomal adhesion gradually becomes calcium-independent and this is associated with an increase in the strength of intercellular adhesion (hyper-adhesion). In this study, we investigated the functional and molecular significance of hyper-adhesion in a system challenged by autoim...

journal_title：Experimental cell research

authors： Cirillo N,Lanza A,Prime SS

更新日期：2010-02-15 00:00:00

abstract：:In this study, we demonstrate that protein kinase C (PKC) activators, including phorbol-12-myristate-13-acetate (PMA), 1,2-dioctanoyl-sn-glycerol (DOG), and platelet-derived growth factor alpha are potent inducers of angiopoietin-like protein 4 (ANGPTL4) expression in several normal lung cell types and carcinoma cell ...

journal_title：Experimental cell research

authors： Stapleton CM,Joo JH,Kim YS,Liao G,Panettieri RA Jr,Jetten AM

更新日期：2010-02-15 00:00:00

abstract：:The abundant heterogeneous nuclear ribonucleoprotein M (hnRNP M) is able to associate with early spliceosomes and to influence splicing patterns of specific pre-mRNAs. Here, by a combination of immunoprecipitation and pull-down assays, we have identified PSF (polypyrimidine tract-binding protein-associated splicing fa...

journal_title：Experimental cell research

authors： Marko M,Leichter M,Patrinou-Georgoula M,Guialis A

更新日期：2010-02-01 00:00:00

abstract：:Tumor endothelial marker (TEM) 5 is an adhesion G-protein-coupled receptor upregulated in endothelial cells during tumor and physiologic angiogenesis. So far, the mechanisms leading to upregulation of TEM5 and its function during angiogenesis have not been identified. Here, we report that TEM5 expression in endothelia...

journal_title：Experimental cell research

authors： Vallon M,Rohde F,Janssen KP,Essler M

更新日期：2010-02-01 00:00:00

abstract：:Osteopontin (OPN) is a multifunctional protein implicated in cellular adhesion and migration. Phosphorylation has emerged as a post-translational modification important for certain biological activities of OPN. This study demonstrates that adhesion of isolated neonatal rat osteoclasts in vitro was augmented on bovine ...

journal_title：Experimental cell research

authors： Ek-Rylander B,Andersson G

更新日期：2010-02-01 00:00:00

abstract：:Myosin phosphatase target subunit 1 (MYPT1), together with catalytic subunit of type1 delta isoform (PP1cdelta) and a small 20-kDa regulatory unit (M20), form a heterotrimeric holoenzyme, myosin phosphatase (MP), which is responsible for regulating the extent of myosin light chain phosphorylation. Here we report the i...

journal_title：Experimental cell research

authors： Twomey E,Li Y,Lei J,Sodja C,Ribecco-Lutkiewicz M,Smith B,Fang H,Bani-Yaghoub M,McKinnell I,Sikorska M

更新日期：2010-01-01 00:00:00

abstract：:VEGF is a major inducer of angiogenesis. However, the homing role of VEGF for cardiac stem cells (CSCs) is unclear. In in vitro experiments, CSCs were isolated from the rat hearts, and a cellular migration assay was performed using a 24-well transwell system. VEGF induced CSC migration in a concentration-dependent man...

journal_title：Experimental cell research

authors： Tang J,Wang J,Kong X,Yang J,Guo L,Zheng F,Zhang L,Huang Y,Wan Y

更新日期：2009-12-10 00:00:00

abstract：:Centromeres are the chromosomal loci that direct the formation of the kinetochores. These macromolecular assemblies mediate the interaction between chromosomes and spindle microtubules and thereby power chromosome movement during cell division. They are also the sites of extensive regulation of the chromosome segregat...

journal_title：Experimental cell research

authors： Bernad R,Sánchez P,Losada A

更新日期：2009-11-15 00:00:00

abstract：:The microtubule cytoskeleton is known to play a role in cell structure and serve as a scaffold for a variety of active molecules in processes as diverse as motility and cell division. The literature on the role of microtubules in signal transduction, however, is marked by inconsistencies. We have investigated a well-s...

journal_title：Experimental cell research

authors： Jackman RW,Rhoads MG,Cornwell E,Kandarian SC

更新日期：2009-11-15 00:00:00

abstract：UNLABELLED:The success of hepatocyte transplantation has been limited by the low efficiency of transplanted cell integration into liver parenchyma. Human fetal hepatic progenitor cells (hepatoblasts) engraft more effectively than adult hepatocytes in mouse livers. However, the signals required for their integration are...

journal_title：Experimental cell research

authors： Delgado JP,Vanneaux V,Branger J,Touboul T,Sentilhes L,Mainot S,Lainas P,Leclerc P,Uzan G,Mahieu-Caputo D,Weber A

更新日期：2009-11-15 00:00:00

abstract：:Celiac disease (CD) is a multisystemic autoimmune inflammation of the intestinal tract induced by wheat gluten and related cereals in HLA-DQ2/8 positive individuals. An essential role in the pathogenesis of CD is played by a fraction of the peptic-tryptic digest of gluten, Frazer's Fraction (FF). Here, we investigate ...

journal_title：Experimental cell research

authors： Reinke Y,Zimmer KP,Naim HY

更新日期：2009-11-15 00:00:00

abstract：:Neuromedin B (NMB) is one of the bombesin-like peptides in mammals. Recently, bombesin-like peptides have been characterized as growth factors in highly vascularized tumors. In this study, we report that NMB potently stimulates in vivo neovascularization in a mouse Matrigel plug and the sprouting of endothelial cells ...

journal_title：Experimental cell research

authors： Park HJ,Kim SR,Bae SK,Choi YK,Bae YH,Kim EC,Kim WJ,Jang HO,Yun I,Kim YM,Bae MK

更新日期：2009-11-15 00:00:00

abstract：:Mip130/LIN-9 is part of a large complex that includes homologs of the Drosophila dREAM (drosophila RB-like, E2F, and Myb) and C. elegans DRM complexes. This complex also includes proteins such as Mip40/LIN-37, Mip120/LIN-54, and LIN-52. In mammalian cells, Mip130/LIN-9 specifically associates with the p107/p130-E2F4 r...

journal_title：Experimental cell research

authors： Sandoval R,Pilkinton M,Colamonici OR

更新日期：2009-10-15 00:00:00

abstract：:Wnt proteins are important regulators of embryonic development, and dysregulated Wnt signalling is involved in the oncogenesis of several human cancers. Our knowledge of the downstream target genes is limited, however. We used a chromatin immunoprecipitation-based assay to isolate and characterize the actual gene segm...

journal_title：Experimental cell research

authors： Railo A,Pajunen A,Itäranta P,Naillat F,Vuoristo J,Kilpeläinen P,Vainio S

更新日期：2009-10-01 00:00:00

abstract：:The nucleoside analogs 9-beta-D-arabinofuranosylguanine (araG) and 1-beta-d-arabinofuranosylthymine (araT) are substrates of mitochondrial nucleoside kinases and have previously been shown to be predominantly incorporated into mtDNA of cells, but the pharmacological importance of their accumulation in mtDNA is not kno...

journal_title：Experimental cell research

authors： Curbo S,Johansson M,Balzarini J,Lewis LD,Karlsson A

更新日期：2009-09-10 00:00:00

abstract：:Podosome formation in vascular smooth muscle cells is characterized by the recruitment of AFAP-110, p190RhoGAP, and cortactin, which have specific roles in Src activation, local down-regulation of RhoA activity, and actin polymerization, respectively. However, the molecular mechanism that underlies their specific recr...

journal_title：Experimental cell research

authors： Crimaldi L,Courtneidge SA,Gimona M

更新日期：2009-09-10 00:00:00

abstract：:Oncogenic EGFRvIII is a naturally occurring oncoprotein and is expressed in about 40-50% of human glioblastomas, particularly those that arise de novo. To understand the molecular mechanisms by which this oncoprotein alters transforming phenotypes, and since our previous work indicated that SHP-2 protein tyrosine phos...

journal_title：Experimental cell research

authors： Zhan Y,Counelis GJ,O'Rourke DM

更新日期：2009-08-15 00:00:00

abstract：:Viral vectors hold promise and challenges in gene therapy. Specifically, we have previously shown that baculoviral (BV) vectors have a high efficiency of gene delivery in human embryonic stem (ES) cells. Here we report the development of a complementary system to further our evaluation by utilizing the laboratory fish...

journal_title：Experimental cell research

authors： Yan Y,Du J,Chen T,Yi M,Li M,Wang S,Li CM,Hong Y

更新日期：2009-08-01 00:00:00

abstract：:RNAi mediated loss of Drp1 function changes mitochondrial morphology in cultured HeLa and HUVEC cells by shifting the balance of mitochondrial fission and fusion towards unopposed fusion. Over time, inhibition of Drp1 expression results in the formation of a highly branched mitochondrial network along with "bulge"-lik...

journal_title：Experimental cell research

authors： Möpert K,Hajek P,Frank S,Chen C,Kaufmann J,Santel A

更新日期：2009-08-01 00:00:00

abstract：:JANEX-1/WHI-P131, a selective Janus kinase 3 (JAK3) inhibitor, has been shown to delay the onset of diabetes in the NOD mouse model. However, the molecular mechanism by which JANEX-1 protects pancreatic beta-cells is unknown. In the current study, we investigated the role of JANEX-1 on interleukin (IL)-1beta and inter...

journal_title：Experimental cell research

authors： Lv N,Kim EK,Song MY,Choi HN,Moon WS,Park SJ,Park JW,Kwon KB,Park BH

更新日期：2009-07-15 00:00:00

abstract：:Cytokeratin 19 (CK19) is widely used as a biomarker for the detection of disseminated tumor cells in blood and bone marrow, and its positivity is considered as an independent prognostication indicator in cancer patients. However, its role in breast cancer progression remains unknown. We had established a stable CK19-e...

journal_title：Experimental cell research

authors： Bambang IF,Lu D,Li H,Chiu LL,Lau QC,Koay E,Zhang D

更新日期：2009-07-01 00:00:00

abstract：:Translational control is an essential process in regulation of gene expression, which occurs at the initiation step performed by a number of translation initiation factor complexes. eIF3a (eIF3 p170) is the largest subunit of the eIF3 complex. eIF3a has been suggested to play roles in regulating translation of a subse...

journal_title：Experimental cell research

authors： Dong Z,Liu Z,Cui P,Pincheira R,Yang Y,Liu J,Zhang JT

更新日期：2009-07-01 00:00:00

abstract：:The majority of human tumors are angiogenesis dependent. Understanding the specific mechanisms that contribute to angiogenesis may offer the best approach to develop therapies to inhibit angiogenesis in cancer. Endothelial monocyte activating polypeptide-II (EMAP-II) is an anti-angiogenic cytokine with potent effects ...

journal_title：Experimental cell research

authors： Tandle AT,Calvani M,Uranchimeg B,Zahavi D,Melillo G,Libutti SK

更新日期：2009-07-01 00:00:00

abstract：:Multiple myeloma is characterized by the malignant growth of immunoglobulin producing plasma cells, predominantly in the bone marrow. The effects of primary human mesenchymal stromal cells on the differentiation phenotype of multiple myeloma cells were studied by co-culture experiments. The incubation of multiple myel...

journal_title：Experimental cell research

authors： Dezorella N,Pevsner-Fischer M,Deutsch V,Kay S,Baron S,Stern R,Tavor S,Nagler A,Naparstek E,Zipori D,Katz BZ

更新日期：2009-07-01 00:00:00